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Introduction
Major urinary protein 1 (Mup1), also known as Mup7, Up-1, Ltn-1, Mup-1, Mup-a, Mup10 and Lvtn-1, is a low molecular weight
secreted protein produced predominantly from the liver. Structurally it belongs to the lipocalin family, which carries small hydrophobic
ligands such as pheromones. Mup1 is an important player in regulating energy expenditure and metabolism in mice, and its
deficiency might contribute to the metabolic dysregulation in obese/diabetic mice.
Description
Total 190 AA. Mw: 22 KDa (calculated). N-terminal His-tag and TEV cleavage site, with 28 extra AA (highlighted).
Amino Acid Sequence
MSYYHHHHHHDYDIPTTENLYFQGAMGSEEASSTGRNFNVEKINGEWHTIILASDKREKIEDNGNFRLFLEQIHVLE
NSLVLKFHTVRDEECSELSMVADKTEKAGEYSVTYDGFNTFTIPKTDYDNFLMAHLINEKDGETFQLMGLYGREPDL
SSDIKERFAQLCEKHGILRENIIDLSNANRCLQARE
Formulation
Lyophilized in PBS.
Reconstitution
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
Storage
Store lyophilized protein at -20°C. Aliquot reconstituted protein and store at -80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Application
ELISA and Western Blotting.
Endotoxin Level
<0.01EU per 1µg of protein by LAL method.