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Introduction
Similar to Kit ligand precursor (C-kit ligand), also known as Stem cell factor (SCF), Mast cell growth factor (MGF), or Hematopoietic
growth factor KL. SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic
factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration.
In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. SCF/C-kit ligand stimulates
the proliferation of mast cells. This protein can augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in
bone marrow culture. It may act synergistically with other cytokines, probably interleukins SCF/C-kit ligand is the ligand for the
tyrosine kinase receptor c-kit, which is expressed on both primitive and mature hematopoietic progenitor cells. In vitro, SCF/C-kit
ligand synergizes with other growth factors, such as granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-
stimulating factor, and interleukin-3 to stimulate the proliferation and differentiation of cells of the lymphoid, myeloid, erythroid, and
megakaryocytic lineages. In vivo, SCF/C-kit also synergizes with other growth factors and has been shown to enhance the
mobilization of peripheral blood progenitor cells in combination with G-CSF. In phase I/II clinical studies administration of the
combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone.
Description
A DNA sequence encoding the amino acid (Met 1- Ala 189) of human SCF (P21583-1) extracellular domain was expressed, with a
polyhistidine tag at the C-terminus. Predicted N terminal: Glu 26.
MW: 19.9 kDa (Calculated)
The apparent molecular mass of rh SCF is approximately 22 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 5% trehalose, 5% mannitol and 0.01% tween-80.
Reconstitution
It is recommended that sterile water (400 ul) be added to the vial to prepare a stock solution of 0.25 mg/mL
Storage
Samples are stable for twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C upon
receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.
Endotoxin Level
< 1.0 EU per μg of the protein as determined by the LAL method
Application
Cell culture, animal studies, ELISA and Western blotting.