Annexin A8 (ANXA8)

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Tag: N-terminal 6xHis

Size: 0.1 mg

Source: E.coli

Purity: >90%

Other Names: ANXA8

Species: Human

Introduction
Annexin A8 (ANXA8) belongs to the annexin family of evolutionally conversed Ca²⁺ and phospholipid binding proteins. ANXA8 may
function as an anticoagulant which inhibits the function of thromboplastin- specific complex in an indirect way. Overexpression of this
gene has been shown to be associated with acute myelocytic leukemia. Human ANXA8 shares 92.661% sequence identity with rat
ANXA8 and 92.094% with mouse ANXA8.

Description

Expressed in E.coli cells with total 392 AA. Mw: 44.35 KDa (calculated).

N-terminal 6xHis-tag, EK and TEV cleavage site, 44 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

Amino Acid Sequence

MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSLEENLYFQGAAWWKSWIEQEGVTVKSSSHFNPDPD
AETLYKAMETKGIGTNEQAIIDVLTKRSNTQRQQIAKSFKAQFGKDLTETLKSELSGKFERLIVALMETYPPYRYEAKE
LHDAMETKGLGTKEGVIIEILASRTKNQLREIMETKAYEEDYGSSLEEDIQADTSGYLERILVCLLQGSRDDVSSFVDP
GLALQDAQDLYAAGEKIRGTDEMETKFITILCTRSATHLLRVFEEYEKIANKSIEDSIKSETHGSLEEAMETLTVVKCTQ
NLHSYFAERLYYAMETKGAGTRDGTLIRNIVSRSEIDLNLIKCHFKKMETYGKTLSSMETIMETEDTSGDYKNALLSLVGSDP

Formulation

Lyophilized at 1mg/mL in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

Storage

Store lyophilized protein at -20°C. Aliquot reconstituted protein and store at -80°C. Avoid repeated freezing/thawing cycles.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Application

ELISA and Western Blotting.

Endotoxin Level

<0.2 EU per 1 μg of the protein by the LAL method.