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Autotaxin (ATX, ENPP2) is a secreted glycoprotein with phosphodiesterase (PDE) activity. It is one of the member in the nucleotide
pyrophosphatase/phosphodiesterase (NPPs) family. ATX has lysophospholipase D activity that converts lysophosphatidylcholine
into LPA, and it was originally identified as a tumor cell-motility-stimulating factor. LPA, which specifically binds to G protein-coupled
receptors (GPCR), plays a wide range of biological activities, including cell hyperproliferation, which may contribute to oncogenesis and metastasis. ATX has been to be overexpressed in many tumor cells, and thus plays an important role in tumor development and
metastasis. In addition, ATX also plays an important role in nervous and immune systems.
Description
Expressed in HEK293 cells with total 871 AA. Mw: 100.1 KDa (calculated).
C-terminal 6xHis-tag, 9 extra AA (highlighted).
Recombinant protein for research use or manufacturing only.
Amino Acid Sequence
MARQGCFGSYQVISLFTFAIGVNLCLGFTASRIKRAEWDEGPPTVLSDSPWTNTSGSCKGRCFELQEVGPPDCRCD
NLCKSYSSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLSRGDCCTNYQVVCKGESHWVDDDCEE
IRVPECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHAPYMRPVYPTKTFPNLYTLATGLYPESHGIVG
NSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVRAGTFFWSVSIPHERRILTILQWLSLPDNERPSVYAFY
SEQPDFSGHKYGPFGPEMTNPLREIDKTVGQLMDGLKQLKLHRCVNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDIT
LVPGTLGRIRPKIPNNLKYDPKAIIANLTCKKPDQHFKPYMKQHLPKRLHYANNRRIEDLHLLVERRWHVARKPLDVYK
KPSGKCFFQGDHGFDNKVNSMQTVFVGYGPTFKYRTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTF
RPTLPEEVSRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLEELNKRLHTKGSTEERHLLYGRPAVLYRTSYDILYHTDFE
SGYSEIFLMPLWTSYTISKQAEVSSIPEHLTNCVRPDVRVSPGFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAF
LVTNMVPMYPAFKRVWTYFQRVLVKKYASERNGVNVISGPIFDYNYNGLRDIEDEIKQYVEGSSIPVPTHYYSIITSCLD
FTQPADKCDGPLSVSSFILPHRPDNDESCNSSEDESKWVEELMKMHTARVRDIEHLTGLDFYRKTSRSYSEILTLKTY
LHTYESEI SYYHHHHHH
Formulation
Lyophilized at 1 mg/mL in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).
Reconstitution
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
Storage
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Application
ELISA and Western Blotting.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Bioactivity Test
Measured by enzymatic ability to cleave Bis (p- Nitrophenyl) Phosphate (BPNPP).
The specific activity of mouse ATX is > 5,000 pmol/min/μg
* Specific Activity (pmol/min/µg) = Adjusted Vmax* (OD/min) x Conversion Factor (pmol/OD)/Amount of Enzyme (µg)