N-terminal B-type Natriuretic Peptide (NT-proBNP)

Origin: Recombinant	Cat. No.: 41660
Tag: N-terminal 6xHis	Size: 0.1 mg
Source: E.coli	Purity: >95%
Other names: Amino terminal brain natriuretic peptide Precursor	Species: Human

Introduction

N-terminal pro-brain (or B-type) natriuretic peptide (NT-proBNP) is produced predominately by the cardiac ventricular myocytes. NT-proBNP is released in response to volume expansion and filling pressure and is involved in maintaining intravascular volume
homeostasis. Elevated plasma levels of BNP and NT-proBNP have been observed at times of cardiac stress and damage.

Description

Expressed in E.coli with total 112 AA. Mw: 12.6 KDa (calculated).

N-terminal 6xHis-tag and TEV cleavage site, 36 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

Amino Acid Sequence

MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSHPLGSPGSASDLETSGLQEQRNHLQGKLSELQ
VEQTSLEPLQESPRPTGVWKSREVATEGIRGHRKMVLYTLRAPR

Formulation

Lyophilized at 1 mg/mL in PBS.

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolves completely.

Storage

Store lyophilized protein at -20°C. Aliquot reconstituted protein and store at -80°C. Avoid repeated freezing/thawing cycles.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Application

Standard ELISA test, Western Blot.

Endotoxin Level

<0.2 EU/μg

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N-terminal B-type Natriuretic Peptide (NT-proBNP)