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Introduction
Procalcitonin is a prohormone of calcitonin. Levels of unprocessed procalcitonin rise significantly after bacterial infection, trauma or
shock. Procalcitonin is also associated with systemic complications in humans suffering from acute pancreatitis.
Description
Expressed in E.coli with total 152 AA. Mw: 16.9 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 36 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
Amino Acid Sequence
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSAPFRSALESSPADPATLSEDEARLLLAALVQDYVQMKAS
ELEQEQEREGSSLDSPRSKRCGNLSTCMLGTYTQDFNKFHTFPQTAIGVGAPGKKRDMSSDLERDHRPHVSMPQNAN
Formulation
Lyophilized at 1 mg/mL in PBS.
Reconstitution
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolves completely.
Storage
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Application
Standard ELISA test, Western Blot.
Endotoxin Level
<0.2 EU/μg