Monoclonal Antibody against Human Autotaxin (Clone 1G7)
Size: 0.1 mg
Introduction
Autotaxin (ATX, ENPP2) is a secreted glycoprotein with phosphodiesterase (PDE) activity. It is one of the members in the nucleotide pyrophosphatase/ phosphodiesterase family (NPPs) family. ATX has lysophospholipase D activity that converts lysophosphatidylcholine into LPA, and it was originally identified as a tumor cell-motility-stimulating factor. LPA, which specifically binds to G protein-coupled receptors (GPCR), has a wide range of biological activities, including cell hyperproliferation, which may contribute to oncogenesis and metastasis. ATX has been shown to be overexpressed in many tumor cells, and thus plays an important role in tumor development and metastasis. In addition, ATX also plays an important role in nervous and immune systems.
Purification
Protein G affinity purification
Immunogen
Recombinant human autotaxin (Cat. No.: 41770) in mammalian cells.
Specificity
The antibody detects human autotaxin in western blot and ELISA.
Formulation
Liquid in phosphate-buffered saline (PBS).
Storage
Store at –20°C for less than one week. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.
Application/Usage
Western blot - This antibody can be used at 3-5 μg/mL with the appropriate secondary reagents to detect human autotaxin.
ELISA - This antibody can be used as a capture antibody in a human autotaxin ELISA in combination with polyclonal anti-human autotaxin antibody as detection antibody.