Human PLAC9 ELISA kit

Cat No.: 31990                                 ★Download Datasheet★

Introduction

Placenta-specific protein 9 (PLAC9) is a putative secreted protein highly enriched in placenta. It plays a major role in embryonic
development. During human embryogenesis, PLAC9 levels were upregulated at 8-9 weeks. PLAC9 was found to be involved in
protein interactions in human liver. Overexpression of PLAC9 inhibited cell proliferation capacity in hepatic cells by modifying cell
cycle related proteins.

Principle of the Assay

This assay is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA). The microtiter plate is pre-coated with a rabbit
polyclonal antibody specific for human PLAC9. Standards and samples are pipetted into the wells and any human PLAC9 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for
human PLAC9 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is
added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human
PLAC9 bound initially. The assay is stopped, and the optical density of the wells determined using a microplate reader. Since the
increases in absorbance are directly proportional to the amount of captured human PLAC9, the unknown sample concentration can
be interpolated from a reference curve included in each assay.

Assay Performance

A. Typical representation of standard curve

The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.