Human Lipocalin-2 ELISA Kit

Cat No.: 31050                                 ★Download Datasheet★

Other Names: LCN2, NGAL, neutrophil lipocalin (NL), 24p3, siderocalin, MSFI

Introduction

Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), 24p3, siderocalin, or neutrophil lipocalin (NL), is a
secretory glycoprotein expressed in liver, lung, kidney, adipocytes, activated neutrophils and macrophages.
Lipocalin-2 is upregulated under various inflammation and infection conditions and can be served as a sensitive biomarker for
various renal injuries. Serum levels of lipocalin-2 are positively correlated with obesity, insulin resistance, hyperglycemia, coronary
heart disease and fatty liver disease in humans.

Principle of the Assay

This assay is a quantitative sandwich ELISA using monoclonal antibodies against human LCN2. The immunoplate is pre-coated with a monoclonal antibody specific for human LCN2. Standards and samples are added into the wells and any human LCN2 present is
captured by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked
monoclonal antibody specific for human LCN2 is added to the wells. After a final washing step to remove any unbound reagents, a
HRP substrate solution is added and colour develops in proportion to the amount of human LCN2 bound initially. The reaction is
stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly
proportional to the amount of captured human LCN2, the unknown sample concentration can be interpolated from a reference curve
included in each assay.

Assay Performance

A. Typical representation of standard curve

The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.

 B. Sensitivity

The lowest level of lipocalin-2 that can be detected by this assay is 0.39 ng/mL.

C. Specificity

The antibody pair used in this assay is specific to human lipocalin-2 and does not cross-react with mouse and rat lipocalin-2, and
other cytokine or hormone molecules.

Publications Citing This Product

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2. Xiao Y, Xu A, Hui X, Zhou P, Li X, Zhong H, Tang W, Huang G, Zhou Z. Circulating lipocalin-2 and retinol-binding protein 4 are
associated with intima-media thickness and subclinical atherosclerosis in patients with type 2 diabetes. PLoS One. 2013 Jun 17;8(6):e66607.
3. Heilbronn LK, Campbell LV, Xu A, Samocha-Bonet D. Metabolically protective cytokines adiponectin and fibroblast growth
factor-21 are increased by acute overfeeding in healthy humans. PloS one. 2013 Oct 18;8(10):e78864.
4. Ni J, Ma X, Zhou M, Pan X, Tang J, Hao Y, Lu Z, Gao M, Bao Y, Jia W. Serum lipocalin-2 levels positively correlate with coronary
artery disease and metabolic syndrome. Cardiovascular diabetology. 2013 Dec;12(1):1-7.
5. Wu G, Li H, Fang Q, Jiang S, Zhang L, Zhang J, Hou X, Lu J, Bao Y, Xu A, Jia W. Elevated circulating lipocalin-2 levels
independently predict incident cardiovascular events in men in a population-based cohort. Arteriosclerosis, thrombosis, and
vascular biology. 2014 Nov;34(11):2457-64.
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Phenotypic characterization of insulin-resistant and insulin-sensitive obesity. The Journal of Clinical Endocrinology & Metabolism.
2015 Nov 1;100(11):4082-91.
7. Na GY, Yoon SR, An J, Yeo R, Song J, Jo MN, Han S, Kim OY. The relationship between circulating neutrophil gelatinase-
associated lipocalin and early alteration of metabolic parameters is associated with dietary saturated fat intake in non-diabetic
Korean women. Endocrine journal. 2016:EJ16-0233.
8. Luo Y, Ma X, Pan X, Xu Y, Xiong Q, Xiao Y, Bao Y, Jia W. Serum lipocalin-2 levels are positively associated with not only total body fat but also visceral fat area in Chinese men. Medicine. 2016 Jul;95(30).
9. Chen DL, Brown R, Liess C, Poljak A, Xu A, Zhang J, Trenell M, Jenkins A, Chisholm D, Samocha-Bonet D, Macefield VG. Muscle sympathetic nerve activity is associated with liver insulin sensitivity in obese non-diabetic men. Frontiers in physiology. 2017 Feb 28;8:101.
10. Yang K, Deng HB, Man AW, Song E, Zhang J, Luo C, Cheung BM, Yuen KY, Jensen PS, Irmukhamedov A, Elie AG. Measuring
non‐polyaminated lipocalin‐2 for cardiometabolic risk assessment. ESC heart failure. 2017 Nov;4(4):563-75.
11. Sun WY, Bai B, Luo C, Yang K, Li D, Wu D, Félétou M, Villeneuve N, Zhou Y, Yang J, Xu A. Lipocalin-2 derived from adipose
tissue mediates aldosterone-induced renal injury. JCI insight. 2018 Sep 6;3(17).
12. Wong YK, Cheung CY, Tang CS, Au KW, Hai JS, Lee CH, Lau KK, Cheung BM, Sham PC, Xu A, Lam KS. Age-biomarkers-
clinical risk factors for prediction of cardiovascular events in patients with coronary artery disease. Arteriosclerosis, thrombosis, and
vascular biology. 2018 Oct;38(10):2519-27.
13. Elie AG, Bloksgaard M, Sun WY, Yang K, Man AW, Xu A, Irmukhamedov A, Riber LP, Wang Y, De Mey JG. Local enrichment of
fatty acid-binding protein 4 in the pericardial cavity of cardiovascular disease patients. PloS one. 2018 Nov 5;13(11):e0206802.
14. Xu Y, Ma X, Pan X, He X, Xiao Y, Bao Y. Correlations between serum concentration of three bone-derived factors and obesity
and visceral fat accumulation in a cohort of middle aged men and women. Cardiovascular diabetology. 2018 Dec;17(1):1-9.
15. Xiao Y, Xiao X, Xu A, Chen X, Tang W, Zhou Z. Circulating adipocyte fatty acid-binding protein levels predict the development of subclinical atherosclerosis in type 2 diabetes. Journal of Diabetes and its Complications. 2018 Dec 1;32(12):1100-4.
16. Tang A, Coster AC, Tonks KT, Heilbronn LK, Pocock N, Purtell L, Govendir M, Blythe J, Zhang J, Xu A, Chisholm DJ. Longitudinal changes in insulin resistance in normal weight, overweight and obese individuals. Journal of clinical medicine. 2019 May;8(5):623.
17. Wu L, Qian L, Zhang L, Zhang J, Zhou J, Li Y, Hou X, Fang Q, Li H, Jia W. Fibroblast Growth Factor 21 is Related to
Atherosclerosis Independent of Nonalcoholic Fatty Liver Disease and Predicts Atherosclerotic Cardiovascular Events. Journal of the American Heart Association. 2020 Jun 2;9(11):e015226.
18. Harari A, Coster AC, Jenkins A, Xu A, Greenfield JR, Harats D, Shaish A, Samocha-Bonet D. Obesity and insulin resistance are
inversely associated with serum and adipose tissue carotenoid concentrations in adults. The Journal of nutrition. 2020 Jan 1;150(1):
38-46.
19. Ong KL, Wu L, Januszewski AS, O'Connell RL, Xu A, Rye KA, Ma RC, Li H, Jenkins AJ, Jia W, Keech AC. Relationships of
adipocyte-fatty acid binding protein and lipocalin 2 with risk factors and chronic complications in type 2 diabetes and effects of
fenofibrate: A fenofibrate Intervention and event lowering in diabetes sub-study. Diabetes Research and Clinical Practice. 2020 Nov
1;169:108450.
20. Botta A, Forest A, Daneault C, Pantopoulos K, Tantiworawit A, Phrommintikul A, Chattipakorn S, Chattipakorn N, Des Rosiers C, Sweeney G. Identification of Circulating Endocan-1 and Ether Phospholipids as Biomarkers for Complications in Thalassemia
Patients. Metabolites. 2021 Feb;11(2):70.
21. Cheng F, Ng NY, Tam CH, Zhang Y, Lim CK, Jiang G, Ng AC, Yau TT, Cheung LP, Xu A, Chan JC. Association between FGF19,
FGF21 and lipocalin-2, and diabetes progression in PCOS. Endocrine connections. 2021 Oct 1;10(10):1243-52.