Cat No.: 31300 Other Names: Myeloblastin, p29b, PRTN3, NP-4
Introduction
Proteinase 3 (PR3), also known as myeloblastin, azurophil granule protein-7 or p29b, PRTN3 and NP- 4, is one of the hematopoietic serine proteases localized in the primary granules of polymorphonuclear neutrophils (PMNs). It is a 29kDa glycoprotein made of 222 amino acids and is released during neutrophilic inflammation.
Principle of the Assay
This assay is a quantitative sandwich ELISA. The microtiter strips are pre-coated with a polyclonal antibody specific for human PR3. Standards and samples are pipetted into the wells and any human PR3 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidise (HRP) labelled polyclonal antibody specific for human PR3 is added to the wells. After wash step to remove any unbound reagents, an HRP substrate solution is added and colour develops in proportion to the amount of human PR3 bound initially. The assay is stopped and the optical density of the wells is determined using a micro-plate
reader. Since the increase in absorbance is directly proportional to the amount of captured human PR3, the unknown sample
concentration can be interpolated from a reference curve included in each assay.
Assay Performance
A. Typical representation of standard curve
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample
assay.
PR3 (ng/mL) | Absorbance (450 nm) | Blanked Absorbance |
0 | 0.056 | 0 |
0.156 | 0.102 | 0.046 |
0.312 | 0.145 | 0.089 |
0.625 | 0.245 | 0.189 |
1.25 | 0.426 | 0.37 |
2.5 | 0.808 | 0.752 |
5 | 1.482 | 1.426 |
10 | 2.603 | 2.54 |
Publications Citing This Product
1. Elie AG, Bloksgaard M, Sun WY, Yang K, Man AW, Xu A, Irmukhamedov A, Riber LP, Wang Y, De Mey JG. Local enrichment of
fatty acid-binding protein 4 in the pericardial cavity of cardiovascular disease patients. PloS one. 2018 Nov 5;13(11):e0206802.
2. Qian LL, Wu L, Zhang L, Zhang J, Zhou J, Li YH, Fang QC, Li HT, Jia WP. Serum biomarkers combined with ultrasonography for
early diagnosis of non-alcoholic fatty liver disease confirmed by magnetic resonance spectroscopy. Acta Pharmacologica Sinica.
2020 Apr;41(4):554-60.
3. Ong KL, Wu L, Januszewski AS, O’Connell R, Xu A, Scott RS, Sullivan DR, Rye KA, Li H, Ma RC, Li L. The relationship of
neutrophil elastase and proteinase 3 with risk factors, and chronic complications in type 2 diabetes: A Fenofibrate Intervention and
Event Lowering in Diabetes (FIELD) sub-study. Diabetes and Vascular Disease Research. 2021 Jul;18(4):14791641211032547.