Cat No.: 31220 Other Names: CRP, PTX1
Introduction
C-reactive protein (CRP) is a circulating protein mainly secreted from the liver. This acute phase protein consists of five identical
non-glycosylated subunits of 23 kDa, that give rise to a symmetrically arranged globular protein with molecular weight of
approximately 120 kDa. It has long been recognized that CRP is closely related to immunology, inflammation and host defence; as a result it has been used as an inflammatory marker. However, the development of high-sensitivity CRP (hsCRP) ELISA had
addressed its role in other clinical issues. There is accumulating evidence suggesting the important role that CRP plays in mediating cardiovascular diseases (CVD) and type 2 diabetes. Normally CRP is presenting only in a trace amount in circulation (<1 µg/ml) but can increase over 1,000-fold under acute inflammatory state. Individual with blood CRP levels
Principle of the Assay
This assay is a quantitative sandwich ELISA using monoclonal antibodies against human CRP. The immunoplate is pre-coated with a monoclonal antibody specific for human CRP. Standards and samples are pipetted into the wells and any human CRP present is
bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked
monoclonal antibody specific for human CRP is added to the wells. After wash step to remove any unbound reagents, an HRP
substrate solution is added and colour develops in proportion to the amount of human CRP bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to
the amount of captured human CRP, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Assay Performance
A. Typical representation of standard curve
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample
assay.
CRP (ng/ml) | Absorbance (450 nm) | Blanked Absorbance |
0 | 0.066 | 0 |
0.312 | 0.125 | 0.059 |
0.625 | 0.183 | 0.117 |
1.25 | 0.317 | 0.251 |
2.5 | 0.545 | 0.479 |
5 | 0.955 | 0.889 |
10 | 1.665 | 1.599 |
20 | 2.498 | 2.432 |
B. Sensitivity
The lowest level of CRP that can be detected by this assay is 0.312 ng/mL.
C. Specificity
The antibody pair used in this assay is specific to human CRP and does not cross-react with mouse and rat CRP, and other cytokine or hormone molecules.
D. Precision
Intra-assay C.V.: 4.3%
Inter-assay C.V.: 5.9%
E. Recovery
The recovery of the assay was determined by adding various amounts CRP to a sample. The measured concentration of the spiked sample in the assay was compared to the expected concentration. The average recovery was 98%.
Publications Citing This Product
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independently predict incident cardiovascular events in men in a population-based cohort. Arteriosclerosis, thrombosis, and vascular biology. 2014 Nov;34(11):2457-64.
2. Chen DL, Liess C, Poljak A, Xu A, Zhang J, Thoma C, Trenell M, Milner B, Jenkins AB, Chisholm DJ, Samocha-Bonet D.
Phenotypic characterization of insulin-resistant and insulin-sensitive obesity. The Journal of Clinical Endocrinology & Metabolism.
2015 Nov 1;100(11):4082-91.
3. Chen DL, Brown R, Liess C, Poljak A, Xu A, Zhang J, Trenell M, Jenkins A, Chisholm D, Samocha-Bonet D, Macefield VG. Muscle sympathetic nerve activity is associated with liver insulin sensitivity in obese non-diabetic men. Frontiers in physiology. 2017 Feb 28;8:101.
4. Yang K, Deng HB, Man AW, Song E, Zhang J, Luo C, Cheung BM, Yuen KY, Jensen PS, Irmukhamedov A, Elie AG. Measuring
non‐polyaminated lipocalin‐2 for cardiometabolic risk assessment. ESC heart failure. 2017 Nov;4(4):563-75.
5. Elie AG, Bloksgaard M, Sun WY, Yang K, Man AW, Xu A, Irmukhamedov A, Riber LP, Wang Y, De Mey JG. Local enrichment of
fatty acid-binding protein 4 in the pericardial cavity of cardiovascular disease patients. PloS one. 2018 Nov 5;13(11):e0206802.
6. Harari A, Coster AC, Jenkins A, Xu A, Greenfield JR, Harats D, Shaish A, Samocha-Bonet D. Obesity and insulin resistance are
inversely associated with serum and adipose tissue carotenoid concentrations in adults. The Journal of nutrition. 2020 Jan 1;150(1):
38-46.
7. Liu Y, Wang Y, Ni Y, Cheung CK, Lam KS, Wang Y, Xia Z, Ye D, Guo J, Tse MA, Panagiotou G. Gut microbiome fermentation
determines the efficacy of exercise for diabetes prevention. Cell metabolism. 2020 Jan 7;31(1):77-91.
8. Lui MM, Mak JC, Chong PW, Lam DC, Ip MS. Circulating adipocyte fatty acid–binding protein is reduced by continuous positive
airway pressure treatment for obstructive sleep apnea—a randomized controlled study. Sleep and Breathing. 2020 Sep;24(3):817-
24.
9. Lui MM, Tse HF, Lam DC, Lau KK, Chan CW, Ip MS. Continuous positive airway pressure improves blood pressure and serum
cardiovascular biomarkers in obstructive sleep apnoea and hypertension. European Respiratory Journal. 2021 Jan 1.