Polyclonal Antibody against Human STAP1
Size: 0.1 mg
Introduction
Signal-transducing adaptor protein 1 (STAP1; also known as BCR downstreamsignaling protein 1, BRDG1) is a 37 kDa adaptor
protein which is involved in B cell antigen receptor signaling. This protein is a substrate of tyrosine-protein kinase Tec, and its
interaction with tyrosine-protein kinase Tec is phosphorylation-dependent. Human STAP1 is 295 amino acids in length and contains
a proline-rich region, a pleckstrin homology (PH) domain, and a region in the carboxy terminal half with similarity to the Src
Homology 2 (SH2) domain. Human STAP1 shares 82% amino acid identity with mouse STAP1.
Purification
Antigen affinity-purified
Immunogen
E. coli derived recombinant human STAP1
Accession # Q9ULZ2
Specificity
The antibody detects human and mouse STAP1 in Western blots.
Formulation
Liquid in phosphate-buffered saline (PBS).
Storage
Store at –20°C for less than one week. For long-term storage, aliquot and freeze at -80°C. Avoid repeated freeze/defrost cycles.
Application/Usage
Please Note: Optimal dilutions should be determined by each laboratory for each application.
Detection of Human BRDG1 by Western Blot.
Western blot shows B cell fractionation of splenic B cells either C57BL/6 STAP1 KO or WT mice. PVDF membrane was probed with 8 µg/mL of Human STAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # 11A121) followed by HRP conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for STAP1 at approximately 37 kDa (as shown in picture).
Western blot – recommended concentration 1~5 ug/ml