Cat No.: 31980 Other Names: MIC-1, PTGF-b, PDF, PLAB, NAG-1, PL741
Introduction
Growth differentiation factor 15 (GDF-15) belongs to transforming growth factor β family1. The biologically active form of GDF-15 is a 28 kDa disulphide linked homodimer2.It is also known as macrophage inhibiting cytokine 1 (MIC-1), placental transformation growth
factor (PTGF-b), prostate derived factor (PDF),placental bone morphogenetic protein (PLAB), NSAID activated gene-1 (NAG-1) and PL741.
Principle of the Assay
This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human GDF-15. Standards and samples are pipetted into the wells and any human GDF-15 present is bound by the immobilized antibody. After
washing away any unbound substances, a biotin labelled polyclonal antibody specific for human GDF-15 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP
substrate solution is added and colour develops in proportion to the amount of human GDF-15 bound initially. The assay is stopped
and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human GDF-15, the unknown sample concentration can be interpolated from a reference curve included
in each assay.
Assay Performance
A. Typical representation of standard curve
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample
assay.
Human GDF-15 (pg/mL) | Absorbance (450 nm) | Blanked Absorbance |
0 | 0.074 | 0 |
7.8 | 0.13 | 0.056 |
15.6 | 0.197 | 0.123 |
31.2 | 0.338 | 0.264 |
62.5 | 0.577 | 0.503 |
125 | 1.017 | 0.943 |
250 | 1.719 | 1.645 |
500 | 2.626 | 2.552 |
B. Sensitivity
The lowest level of human GDF-15 that can be detected by this assay is 7.8 pg/mL.
C. Precision
Intra-assay Precision (Precision within an assay) C.V.<10%.
Inter-assay Precision (Precision between assays) C.V.<10%.
Publications Citing This Product
1. Wu X, Xuan W, You L, Lian H, Li F, Zhang X, Chen Q, Sun K, Chen C, Xu M, Li Y. Associations of GDF-15 and GDF-15/
adiponectin ratio with odds of type 2 diabetes in the Chinese population. Endocrine. 2021 May;72(2):423-36.