Mouse GDF-15 ELISA Kit

Cat No.: 32980                                  Other Names: MIC-1, PTGF-b, PDF, PLAB, NAG-1, PL741

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Introduction

Growth differentiation factor 15 (GDF-15) is a member of the transforming growth factor β cytokine superfamily, the protein is
secreted as a 25 kDa disulfide-linked dimer. Circulating GDF-15 levels are associated with cancers, cardiovascular and kidney
diseases. GDF-15 regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

Principle of the Assay

This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a polyclonal antibody specific for mouse GDF-15. Standards and samples are pipetted into the wells and any mouse GDF-15 present is bound by the immobilized antibody. After
washing away any unbound substances, a biotin labelled polyclonal antibody specific for mouse GDF-15 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP
substrate solution is added and colour develops in proportion to the amount of mouse GDF-15 bound initially. The assay is stopped
and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse GDF-15, the unknown sample concentration can be interpolated from a reference curve included
in each assay.

Assay Performance

A. Typical representation of standard curve

The following standard curve is provided for demonstration only.  A standard curve should be generated for each set of sample
assay. 

B. Sensitivity

 The lowest level of mouse GDF-15 that can be detected by this assay is 3.9 pg/mL.

C. Precision

Intra-assay Precision (Precision within an assay) C.V.<2.9%.

Inter-assay Precision (Precision between assays) C.V.<6.1%.