Cat No.: 32B090 ★Download Datasheet★
Introduction
IL-6 receptor alpha (IL-6Ra) is a transmembrane subunit of the receptor for Interleukin-6. IL-6 is crucial for the development of the
acute phase response, inflammation, hematopoiesis, bone metabolism, and cancer. Acute inflammation is triggered by IL-6, and it
then progresses to either acquired immunity or chronic inflammation. It contributes to inflammatory bowel disease, obesity, insulin
resistance, arthritis, sepsis, and atherosclerosis when it is dysregulated.
Principle of the Assay
This assay is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA). The microtiter plate is pre-coated with a
polyclonal antibody specific for mouse IL-6Ra. Standards and samples are pipetted into the wells and any mouse IL-6Ra present is
bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for
mouse IL-6Ra is added to the wells. After wash step to remove any unbound reagents, streptavidin-horseradish peroxidase
conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and color develops in proportion to the
amount of mouse IL-6Ra bound initially. The assay is stopped, and the optical density of the wells is determined using a microplate
reader. Since the increases in absorbance are directly proportional to the amount of captured mouse IL-6Ra, the unknown sample
concentration can be interpolated from a reference curve included in each assay.
Assay Performance
TYPICAL STANDARD CURVE
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.
Mouse IL-6Ra (pg/mL) | Absorbance (450 nm) | Blanked Absorbance |
0 | 0.128 | 0 |
46.9 | 0.182 | 0.054 |
93.8 | 0.232 | 0.104 |
187.5 | 0.347 | 0.219 |
375 | 0.54 | 0.412 |
750 | 0.89 | 0.762 |
1500 | 1.569 | 1.441 |
3000 | 2.801 | 2.673 |
Mouse IL-6Ra standard curve (4-parameter)
ASSAY CHARACTERISTICS
A. Sensitivity
The lowest level of mouse IL-6Ra that can be detected by this assay is 46.9 pg/mL.
B. Precision
Intra-assay Precision (Precision within an assay) C.V.<3.4%. Inter-assay Precision (Precision between assays) C.V. <6.8%.
Intended Use
This Mouse IL-6Ra ELISA kit is designed for quantification of mouse IL-6Ra in serum, plasma and cell culture supernate samples.