Human Leptin ELISA Kit

Cat No.: 31B030               ★Download Datasheet★

Introduction

Leptin is a product of the mouse obese gene secreted by adipocytes. It controls body fat deposition and energy balance. Leptin
deficiency in humans and mice can result in obesity. Circulating levels of Leptin are regulated by food consumption, insulin levels
and pregnancy status. Mice with leptin synthesis-blocking mutations in the obese gene develop obesity and diabetes as well as
decreased activity, metabolism, and body temperature. 

Principle of the Assay

This assay is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA). The microtiter plate is pre-coated with a
polyclonal antibody specific for human Leptin. Standards and samples are pipetted into the wells and any human Leptin present is
bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for
human Leptin is added to the wells. After wash step to remove any unbound reagents, streptavidin-horseradish peroxidase conjugate
(STP-HRP) is added. After the last wash step, an HRP substrate solution is added and color develops in proportion to the amount
of human Leptin bound initially.  The assay is stopped, and the optical density of the wells is determined using a microplate reader.
Since the increases in absorbance are directly proportional to the amount of captured human Leptin, the unknown sample
concentration can be interpolated from a reference curve included in each assay. 

Assay Performance

TYPICAL STANDARD CURVE

The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.

Human Leptin standard curve (4-parameter)

ASSAY CHARACTERISTICS

A. Sensitivity

The lowest level of human Leptin that can be detected by this assay is 31.25 pg/mL.

B. Precision

Intra-assay Precision (Precision within an assay) C.V.<2.9%.
Inter-assay Precision (Precision between assays) C.V.<6.1%.